Title: The comparison of mackerel tuna and sardines' protein concentration
Objective:
1. To study the protein concentration of mackerel tuna and sardines
2. To study the difference between mackerel tuna and sardines' protein concentration.
3. To study the protein concentration of mackerel tuna and sardines by using Lowry and Biuret method.
Methods
Objective:
1. To study the protein concentration of mackerel tuna and sardines
2. To study the difference between mackerel tuna and sardines' protein concentration.
3. To study the protein concentration of mackerel tuna and sardines by using Lowry and Biuret method.
Methods
![](http://www.weebly.com/weebly/images/file_icons/xls.png)
protein_assay_lab.pptx | |
File Size: | 42 kb |
File Type: | pptx |
Result
Protein concentration
Mackerel tuna
For Biuret is 6.3694mg/mL
For Lowry is 6.41728mg/mL
Sardines
For Biuret is 5.801mg/mL
For Lowry is 4.418mg/mL
Hence the mackerel tuna has more protein concentration than the sardines.
Protein concentration
Mackerel tuna
For Biuret is 6.3694mg/mL
For Lowry is 6.41728mg/mL
Sardines
For Biuret is 5.801mg/mL
For Lowry is 4.418mg/mL
Hence the mackerel tuna has more protein concentration than the sardines.
What have you learned?
1. The equation of M1V1=M2V2 was used in the calculation to get the diluted solution from the stock solution.
2. The standard curve was able to constructed after the data was gained from the carefully conducted experiment.
3. Such lab reflection is more human-easy and suitable for the Biochemistry lab we conducted as degree students, once we get used to it.
4. The protein concentration of a substance can be calculated by using the Biuret and Lowry method.
5. The Lowry method is more sensitive than Biuret method in testing a larger volume of sample.
What do I need to know more from this experiment?
1. The other protein assay which is the Bradford assay can be done to try out the best method for the experiment
2. Protein assay can be done on other fish species to find the best fish with the most protein concentration
3. Protein assay can be done on insect for basic science
1. The equation of M1V1=M2V2 was used in the calculation to get the diluted solution from the stock solution.
2. The standard curve was able to constructed after the data was gained from the carefully conducted experiment.
3. Such lab reflection is more human-easy and suitable for the Biochemistry lab we conducted as degree students, once we get used to it.
4. The protein concentration of a substance can be calculated by using the Biuret and Lowry method.
5. The Lowry method is more sensitive than Biuret method in testing a larger volume of sample.
What do I need to know more from this experiment?
1. The other protein assay which is the Bradford assay can be done to try out the best method for the experiment
2. Protein assay can be done on other fish species to find the best fish with the most protein concentration
3. Protein assay can be done on insect for basic science